Exploring cryo-plasma FIB/SEM volume imaging for biological specimens
In the rapidly evolving field of biological imaging, a recent study has explored the untapped potential of serial cryogenic plasma-focused ion beam scanning electron microscopy (cryo-serial pFIB/SEM) to visualize biological samples with unprecedented clarity and detail. Cryo-serial plasma FIB/SEM represents a leap forward in biological imaging. It allows scientists to examine cells, tissues, and even […]
How to reduce contamination in Cryo-ET with advanced Cryo-FIB techniques
Cryogenic electron tomography (cryo-ET) is a powerful technique that enables researchers to visualize macromolecular complexes within their near-native cellular environment [1,2]. This method has revolutionized the field of structural biology by providing insights into the organization and function of cellular components at near-atomic resolution [3]. However, for cryo-ET to be effective, the sample must be […]
Addressing ice contamination challenges in the Cryo-ET workflow
Cryo-electron tomography (Cryo-ET) is a powerful imaging technique that enables the visualization of biomolecular structures in their near-native state at subnanometer resolutions. By combining a series of 2D images taken at different tilt angles into a 3D reconstruction, cryo-ET provides unparalleled insights into protein-protein interactions and molecular machinery within cells. This powerful technique has revolutionized […]
