Revealing the ultrastructure of autophagic membranes during different stages of biogenesis with correlative cryo-ET
By visualizing membrane ultrastructure directly in the cells, researchers can better understand their diversity, dynamics, and role. In this blog post, we highlight a beautiful example where researchers used cryo-CLEM to reveal the ultrastructural changes in the autophagosome membrane during its biogenesis. Autophagy is a process in which cells maintain their internal homeostasis, by clearing […]
How to choose a suitable camera for cryo-fluorescence light microscopy?
When choosing the most suitable camera for a cryo-fluorescence system to be applied in cryo-electron tomography (cryo-ET), it is important to consider several parameters that will define the fluorescence image quality. In this blog post, we will review the most critical ones and explain how they affect the final results. In cryo-ET, the acquisition of […]
Overcoming artifacts in cryogenic fluorescence microscopy
Cryogenic fluorescence microscopy (cryo-FLM) combined with scanning electron microscopy (SEM) makes the process of lamella milling in the cryo-electron tomography (cryo-ET) workflow more accurate and efficient. The benefit of cryo-FLM can however be compromised if the optical design of the system does not allow the users to easily overcome the artifacts inherent to fluorescence microscopy. […]
Uncovering 3D molecular organization and interactions between muscle proteins using in situ cryo-electron tomography
There are more than 150 known musculoskeletal conditions (1), many of which affect specifically skeletal muscles and can lead to lifelong disabilities or even premature death (2). In order to develop medical treatments, it is critical to understand how healthy and impaired muscles work on the molecular level. In this blog post, we highlight a […]
