Combining light and electron microscopy allows studying the ultrastructure of cells with high-resolution electron microscopy, while simultaneously performing fluorescence imaging to reveal valuable information about cellular function from different parts of the cell. The next step of studying cells is cryo-ET, which can shed light on the connections between different intracellular membrane systems by membrane contact sites.
Identify the region of interest rapidly
Understand how the cell operates within the context of surrounding tissue
Integrated CLEM combines the two imaging methods EM and FM, to offer a powerful streamlined and automated acquisition of high resolution CLEM images in the SEM. The platform is a retro fit compatible with SEM’s from the entire range of manufacturers. In iCLEM, the cells of interest can be identified using FM. Subsequently, a high magnification EM image can be acquired at the desired location to reveal structural details at high resolution. Following this, the two acquired images will be overlayed using the high accuracy automated overlay procedure. It therefore offers seamless CLEM imaging with no sample transfer or manual overlay, providing high quality data in a user-friendly manner, ensuring that you can focus on analyzing data rather than on acquiring it.
Integrating a fluorescence light microscope into the FIB/SEM can optimize the cryo-ET workflow. Using an integrated FLM in the FIB/SEM means that ROI identification by FLM, SEM imaging, FIB milling and ROI confirmation by FLM can all be done in the same system without the need to handle the sample, thus increasing the success rate of the cryo-ET workflow considerably. No need for dangerous and damaging transfer steps.
The integrated CLEM solution allows you to image the cell(s) of interest with both EM and FM in one integrated system, streamlining the workflow and avoiding sample damage and contamination. HeLa cells transfected with GFP-C1 have been imaged to achieve super-resolution molecular localisations of ~ 85 nm, enabling the high-precision correlation of fluorophore-labelled macromolecules to cellular ultrastructure.
Get in touch with our applications specialist Caspar Jonker to get more information.
Call +31 (0)15 744 01 58 or book a meeting on his agenda.
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